Modifying Enzymes
ABclonal Technology Mth RNA Ligase
Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5-phosphorylated single-stranded DNA into 5-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3 end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.
ABclonal Technology Exonuclease VII
Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5-3 and 3-5 directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzymes digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).
ABclonal Technology T5 Exonuclease
T5 Exonuclease is a double-stranded DNA-specific exonuclease and single-stranded DNA endonuclease that degrades DNA in the 5′ ?to 3′ ?direction. T5 Exonuclease can digest nucleotides starting from the 5 end or at gaps and nicks in linear or circular dsDNA. The enzyme does not digest supercoiled dsDNA and exhibits endonuclease activity on ssDNA
ABclonal Technology RNase A (Powder)
Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3 end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3 phosphate and an oligonucleotide with pyrimidine 3 phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein. Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.
ABclonal Technology RNase A (10 mg/mL)
Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3 end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3 phosphate and an oligonucleotide with pyrimidine 3 phosphate at the end. RNase A does not work on DNA. This product is DNase and protease free, and it can be used without heat treatment before use. This product is provided in a solution form at a concentration of 10 mg/mL. The recommended working concentration is 1-100 μg/mL, which varies depending on specific applications.
Agilent Technologies ENDO-O-GLYCOSIDASE
Releases unsubstituted, Ser/Thr-linked GalGalNAc from proteins; used with additional enzymes to remove more complex O-linked structures. Also known asEndo-O-Glycosidase, Endo- N-acetylgalactosaminidase, O-Glycosidase.
ABclonal Technology Exonuclease I (E. coli)
Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA in the 3 to 5 direction, and does not degrade DNA strands with the 3-OH end blocked by phosphoryl or acetyl groups. Exonuclease I has strict substrate specificity and is incapable of degrading double-stranded DNA and RNA.
ABclonal Technology T7 Endonuclease I
T7 Endonuclease I (T7 Endo I, T7EI)，can recognize and cleave non-perfectly mismatched DNA, cruciform DNA structures, holliday structures or junctions and Heteroduplex DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5? end to the mismatch.
ABclonal Technology T3 DNA Ligase
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold). As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.
TRIBIOSCIENCE INC Elastase, 5MG
Product Details: Form: Freeze-dried powder; Solubility: Distilled water or dilute buffer; Stability: Store at -20° C (-4° F); Activity: 10 U/mg protein; Protein: 90%
Amsbio LLC Heparinase III from Recombinant Flavobacterium heparinum - Research Grade
Heparinase III from Recombinant Flavobacterium heparinum - Research Grade
Research Products International Corp Proteinase K, 500 Milligrams
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.
Sigma Aldrich Fine Chemicals Biosciences β-Glucuronidase from Helix pomatia | 9001-45-0 | MFCD00130629 | 5ML
β-Glucuronidase from Helix pomatia | Purity: | Mol Wt: | 9001-45-0 | MFCD00130629 | 5ML