Modifying Enzymes
ACROBiosystems GMP Salt Active GENIUS™Nuclease
GMP Salt Active GENIUS™Nuclease is expressed from Serratia marcescens E. coli.
Carnabio Usa Inc ZAP70/10UG/FULL-LENGTH
ZAP70, Full-length, Wild type, Amino Acid 1-619, NP_001070.2, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc PDHK2(PDK2)/10UG/FULL-LENGTH
PDHK2(PDK2), Full-length, Wild type, Amino Acid 1-407, NP_002602.2, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. Lambda Protein Phosphatase – 20000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
New England Biolabs, Inc. Antarctic Phosphatase – 1000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
New England Biolabs, Inc. Factor Xa Protease – 250 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
Medchemexpress LLC HY-19801 5mg Medchemexpress, CVT-10216 CAS:1005334-57-5 Purity:>98%
Medchemexpress, HY-19801 5mg CVT-10216 CAS:1005334-57-5 CVT-10216 is a highly selective, reversible aldehyde dehydrogenase-2 (ALDH-2) inhibitor with an IC50 of 29 nM. CVT-10216 also has inhibitory effect of ALDH-1 with an IC50 of 1.3 μM. CVT-10216 can reduce excessive alcohol drinking in alcohol-preferring rats and exhibit anxiolytic effects. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Medchemexpress LLC Elastase, Porcine pancreas (EC 3.4.21.36) | 39445-21-1 | 25898.13 | 5 MG
Elastase, Porcine pancreas (EC 3.4.21.36) is a single polypeptide chain of 240 amino acid residues, derived from pig pancreas. It is a serine protease that can hydrolyze proteins and polypeptides. Elastase from porcine pancreas can induce emphysema in hamsters.
- Derived from pig pancreas
- Functions as a serine protease
- Hydrolyzes proteins and polypeptides
- Used in in-solution digestion protocols
- Recommended enzyme:protein ratios of 1:20 to 1:100
- Incubation typically 2-18 hours at 37°C
Enzyme Research Laboratories Inc HUMAN FACTOR 10; 0.8MG
Human Factor X,100 U (0.8 mg),Buffer Composition = 20 mM Tris-HCl,0.1 M NaCl,1 mM Benzamidine,pH 7.4,Extinction Coefficient (1%) = 11.6,One Unit = 8 ug,Molecular Weight = 58,800 daltons,Prepared from fresh frozen human plasma
Apexbio Technology LLC E.-COLI-DNA-LIGASE-200U
E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups